Journal Title
Title of Journal: Trop Anim Health Prod
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Abbravation: Tropical Animal Health and Production
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Publisher
Springer Netherlands
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Authors: Simon Mwangi Kihu George Chege Gitao Lily Caroline Bebora Munene John Njenga Gidraph Gachunga Wairire Ndichu Maingi Raphael Githaiga Wahome Julius Otieno Oyugi Ernest Lutomia
Publish Date: 2014/10/19
Volume: 47, Issue: 1, Pages: 247-249
Abstract
Peste des petits ruminants virus that causes a highly infectious and often fatal disease of sheep and goats is confirmed by various diagnostic techniques among them being isolation of the virus from cell culture systems viral ribonucleic acid RNA detection by molecular assays and viral antigen detection by immunocapture enzymelinked immunosorbent assay IC ELISA immunohistochemistry IHC and AGAR gel test Whereas most of the confirmatory diagnostic procedures require pathological samples to be stored frozen to preserve integrity of the peste des petits ruminants PPR virus RNA samples for IHC tests are preserved in 10 formalin In this study nine formalinfixed pathological samples from three goats suspected of PPR were processed for extraction of PPR viral RNA and analyzed for detection with realtime reverse transcription–polymerase chain reaction qRTPCR assay The results showed that five out of the nine tested samples returned positive for presences PPR viral genome This study has established that field pathological samples of PPRsuspected cases collected and stored in 10 formalin for up 2 years could be used for PPR virus RNA extraction for disease virus confirmationThis is to acknowledge “The Regional Universities Forum for Capacity Building in Agriculture” RUFORUM RU/CFP/CGS/TADS/09/1 for the financial support as well as Vetworks Eastern Africa for their logistical assistance towards this study Acknowledgement also goes to University of Nairobi Institute of Tropical and Infectious Diseases UNITID for providing their molecular laboratories to carry out the molecular analysis of this study which was part of corresponding author’s PhD research workThis field pathological study was conducted in a manner to ensure quality and integrity of the research Consent was sought from Directorate of Veterinary Services who granted the permission for collection of field laboratory samples on peste des petit ruminants vide letter referenced “RefMeat/VolXIV/42” dated 1st July 2011 Consent was also sought from Turkana herders for voluntary presentation of their small stock for collection of samples which they granted and facilitated the exercise
Keywords:
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- Leucaena and dried poultry waste improve the performance of West African Dwarf sheep on a grass diet
- The phenotypic and genotypic characterization of Bacillus anthracis isolates from Iran
- Divergent development of testosterone secretion in male zebu (Bos indicus) and crossbred cattle (Bos indicus x Bos taurus) and buffaloes (Bubalus bubalis) during growth
- Dystocia in Friesian cows and its effects on postpartum reproductive performance and milk production
- Effect of grape seed and skin supplement on milk yield and composition of dairy ewes
- Physiological response of dromedary camels to road transportation in relation to circulating levels of cortisol, thyroid hormones and some serum biochemical parameters
- Improvement of trivalent leptospira vaccine by removal of anaphylactic agents
- Molecular detection of pestiviruses in aborted foetuses from provinces in northern Turkey
- Analysis of production objectives and breeding practices of dairy goats in Kenya
- Effect of feeding sweet sorghum stover-based complete rations on the growth performance and carcass characteristics of ram lambs
- Effect of housing conditions (clean vs. dirty) on growth performance and feeding behavior in growing pigs in a tropical climate
- Prevalence of and risk factors for bovine respiratory syncytial virus (BRSV) infection in non-vaccinated dairy and dual-purpose cattle herds in Ecuador
- Paratuberculosis in buffaloes in Northeast Brazil
- Risks factors associated with subclinical mastitis in water buffaloes in Pakistan
- Effect of adaptation strategies when feeding fresh cassava foliage on intake and physiological responses of lambs
- Genetic and phenotypic parameters and annual trends for milk production and fertility traits of the Sahiwal cattle in semi arid Kenya
- Characterisation of adopters and non-adopters of dairy technologies in Ethiopia and Kenya
- Molecular and pedigree analysis applied to conservation of animal genetic resources: the case of Brazilian Somali hair sheep
- Prevalence, risk factors, and distribution of Cysticercus tenuicollis in visceral organs of slaughtered sheep and goats in central Ethiopia
- Production performance of Dera Din Panah goat under desert range conditions in Pakistan
- The population structure of Salmonella enterica Enteritidis in Iran analyzed by multiple-locus variable-number tandem repeat analysis
- Effects of urea-treated fibrous diets on nutrient intake, digestibility, performance and haematological parameters of Yankasa rams
- Genetic differences in the body weight and haematological traits of Nigerian indigenous chickens infected with Eimeria tenella
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- Comparative analysis of hatching rates and clutch sizes of Nile crocodile ( Crocodylus niloticus ) eggs collected on- and off-farm in Zimbabwe
- A study on the ovarian follicular dynamic in Iraqi Northern Buffaloes
- A survey of decision making practices, educational experiences, and economic performance of two dairy farm populations in Central Thailand
- Toxoplasmosis in camels ( Camelus dromedarius ) of Borana zone, Oromia region, Ethiopia: seroprevalence and risk factors
- Modelling the potential benefits of different strategies to control infection with Trypanosoma evansi in camels in Somaliland
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- Ocular infection of donkeys (Equus asinus) with Setaria equina
- Production and economic performance of F1-crossbred dairy cattle fed non-conventional protein supplements in Zimbabwe
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- Effect of Mycobacterium avium subsp. paratuberculosis (MAP) infection on the diagnostic accuracy for Mycobacterium bovis ( M. bovis ) infection under field conditions in cattle belonging to low M. bovis prevalence herds
- Performance of beef heifers supplemented with sodium lasalocid
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- Formulation of novel nano-encapsulated Newcastle disease vaccine tablets for vaccination of village chickens
- Effect of carbohydrate source and cottonseed meal level in the concentrate: IV. Feed intake, rumen fermentation and milk production in milking cows
- Buffel grass ( Cenchrus ciliaria L.) substitution for orange pulp on intake, digestibility, and performance of hairsheep lambs
- Productivity and parasitic infections of pigs kept under different management systems by smallholder farmers in Mbeya and Mbozi districts, Tanzania
- Seroprevalence and correlates of Toxoplasma gondii infection in domestic pigs in Veracruz State, Mexico
- Animal production systems of small farms in the Kaski district of Nepal
- Effect of resinous extract from Commiphora swynnertonii (Burrt) on experimental coccidial infection in chickens
- Improving productive and reproductive performance of dual-purpose crossbred hens in the tropics by lecithin supplementation
- Prevalence and antimicrobial resistance profiles of Salmonella enterica serovars isolated from slaughtered cattle in Bahir Dar, Ethiopia
- The Damara in the context of Southern Africa fat-tailed sheep breeds
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