Authors: Rainak Ghaderi Keyvan Tadayon Sargis Avagyan Pejvak Khaki Soheila Moradi Bidhendi Ken James Forbes Nader Mosavari Mohammad Reza Toroghi Farhad Moosakhani Reza Banihashemi Mohamad Sekhavati Nasim Karimnasab
Publish Date: 2012/10/25
Volume: 45, Issue: 4, Pages: 889-894
Abstract
Salmonella enterica Enteritidis is the most frequent etiological agent of salmonellosis in humans and poultry To understand the genetic diversity of S Enteritidis in Iran we examined 69 chicken isolates from 18 broiler farms and six nonepidemic human isolates from six geographically distant provinces by multilocus variablenumber tandem repeat analysis MLVA Among SE2 SE3 SE5 SE7 SE8 SENTR4 and SENTR7 only SE5 with four and SENTR7 with two alleles respectively proved variable giving estimates of locus genetic diversity of 058 and 0 In all six closely related MLVA profiles were identified among which three were commonly represented by human and chicken isolates This population homogeneity contrasts with the high diversity at these loci reported elsewhere and is likely a consequence of a single clone of S Enteritidis distributed across IranThis work was fully financed with the state funds from Razi Vaccine and Serum Research Institute under grant no 2181890036 Dr R Firouzi from School of Veterinary Sciences Shiraz University is acknowledged for providing Fars isolates We thank Leila Djose Bayat for laboratory assistance Rainak Ghaderi from RVSRI is a PhD researcher at the Armenian State Agrarian University
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