Molecular and pedigree analysis applied to conserv

Authors: Samuel R Paiva Olivardo Facó Danielle A Faria Thaísa Lacerda Gabriel B Barretto Paulo L S Carneiro Raimundo N B Lobo Concepta McManus

Publish Date: 2011/05/02

Volume: 43, Issue: 7, Pages: 1449-1457

PDF Link

Abstract

The first registers of Somali sheep in Brazil are from the beginning of the 1900s This breed adapted to the dry climate and scarce food supply is restricted in the northeast region of the country Molecular marker technologies especially those based on genotyping microsatellite and mtDNA loci can be used in conjunction with breeding pedigree analysis and consequently the maintenance of genetic variation in herds Animals from the Brazilian Somali Conservation Nuclei from Embrapa Sheep and Goats in Ceará State were used to validate genetic monitoring by traditional pedigree methods and molecular markers Nineteen microsatellite markers and 404 base pairs from the control region of mtDNA were used For total herd diversity an average 532 alleles were found with expected heterozygosity of 05896 observed heterozygosity of 06451 04126 for molecular coancestrality and coefficient of inbreeding F IS was −0095 Comparing molecular coancestrality means over the years there was a consistent increase in this parameter within the herd increasing from 04157 to 04769 in 2 years approx 12 variation Sixteen mtDNA haplotypes were identified Inbreeding and other estimates from genealogical analyses confirm the results from molecular markers From these results it is possible to state that microsatellites are useful tools in genetic management of herds especially when routine herd recording is not carried out or there were gaps in recent generations As well as pedigree control genetic diversity can be optimized Based on the results and despite herd recording in the herd of Brazilian Somali of Embrapa Sheep and Goats additional management measures need to be carried out in this herd to reduce inbreeding and optimize genetic variation

Keywords: