Ameliorating reactive oxygen speciesinduced Emph

Authors: T A Ajith

Publish Date: 2010/02/10

Volume: 25, Issue: 1, Pages: 67-73

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Abstract

Iron is an essential nutrient for a number of cellular activities However excess cellular iron can be toxic by producing reactive oxygen species ROS such as superoxide anion O 2 − and hydroxyl radical HO· that damage proteins lipids and DNA Mutagenic and genotoxic end products of lipid peroxidation can induce the decline of mitochondrial respiration and are associated with various human ailments including aging neurodegenerative disorders cancer etc Zingiber officinale Roscoe ginger is a widely used spice around the world The protective effect of aqueous ethanol extract of Z officinale against ROSinduced in vitro lipid peroxidation and DNA damage was evaluated in this study The lipid peroxidation was induced by hydroxyl radical generated from Fenton’s reaction in rat liver and brain homogenates and mitochondrial fraction isolated from rat liver The DNA protection was evaluated using H2O2induced changes in pBR322 plasmid and Fenton reactioninduced DNA fragmentation in rat liver The results indicated that Z officinale significantly P0001 protected the lipid peroxidation in all the tissue homogenate/mitochondria The extract at 2 and 05 mg/ml could protect 92 of the lipid peroxidation in brain homogenate and liver mitochondria respectively The percent inhibition of lipid peroxidation at 1mg/ml of Z officinale in the liver homogenate was 94 However the extract could partially alleviate the DNA damage The protective mechanism can be correlated to the radical scavenging property of Z officinale The results of the study suggest the possible nutraceutical role of Z officinale against the oxidative stress induced human ailments

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