Authors: Bayan Alsaid Thomas Bessede Djibril Diallo Ibrahim Karam Jean François Uhl Vincent Delmas Stéphane Droupy Gérard Benoît
Publish Date: 2011/07/21
Volume: 34, Issue: 8, Pages: 721-729
Abstract
Classic anatomical methods have limitations in micro determination of nerve fibre location Furthermore the precise detection of the nerve fibres nature is not possible by means of dissection The combination of immunohistochemistry and threedimensional reconstruction could be used to resolve these limitations of morphological sciences Our aim is to describe the evolution of computerassisted anatomic dissection CAAD which is an original method applied to study the distribution of intrapelvic nerves in anatomic researchSerial transverse sectioning of the pelvic region in rabbit human fetus infant and adult cadaver was performed Sections were immunohistochemically stained and digitized with a high optical resolution scanner Photoshop 7 software was used in regrouping of the adult cadaver sections then a tridimensional reconstruction was achieved using WinSurf softwareThe 3D reconstruction of the immunohistochemically stained histologic sections of the pelvis allowed for precise structural identification of the prostate and its innervations in fetus infant and adult In addition we reconstructed the entire intrapelvic organs with accurate demonstration of the location of both adrenergic and cholinergic pathways Moreover we performed a virtual dissection of each of the pelvic structures with description of the exact location of the inferior hypogastric plexus as well as the nature and the distribution of its fibresThe CAAD is an original method in anatomic research which illustrates the fact that descriptive anatomy is still a dynamic science This method allows for a 3D presentation of the intraorganic innervation the nature of the nerve fibres and the distribution of receptors and their neurotransmitters This technique improves the understanding of the complex anatomic regions such as the pelvis from both surgical and educational point of view
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