Authors: MengYun Chou Cary D Austin Jeong M Kim
Publish Date: 2011/12/11
Volume: 61, Issue: 7, Pages: 1055-1064
Abstract
Based on the specificity of antigen recognition and the ability to generate longlived memory responses cancer immunotherapies primarily target tumorassociated T cells Systemic administration of antiIL10R1 antibody in combination with local CpG administration has been shown to induce tumor regression in a Tcelldependent manner Here we confirmed the antitumor efficacy of antiIL10R1 and CpG therapy in the highly aggressive B16F10 melanoma model However T cells were not required for tumor growth inhibition Through cellular depletions and genetic models of leukocyte deficiency we demonstrated that T B and NK cells and neutrophils are not essential for antitumor efficacy Nevertheless hematopoietic cells as a whole are required for antiIL10R1 and CpGinduced tumor growth inhibition suggesting that the collective action of multiple subsets of hematopoieticderived cells is required for antitumor efficacyWe thank Andres PalerMartinez for assistance with Luminex data Wenjun Ouyang for providing il10r1 −/− mice and Ira Mellman for scientific discussions All authors are employees at Genentech a member of the Roche Group The authors declare no additional financial conflicts
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