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Title of Journal: J Membrane Biol

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Abbravation: The Journal of Membrane Biology

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Springer US

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DOI

10.1016/j.amjcard.2009.07.005

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1432-1424

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Biological Properties of Melanoma and Endothelial

Authors: Masa Bosnjak Lara Prosen Tanja Dolinsek Tanja Blagus Bostjan Markelc Maja Cemazar Celine Bouquet Gregor Sersa
Publish Date: 2013/05/07
Volume: 246, Issue: 11, Pages: 803-819
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Abstract

The data on the biological responsiveness of melanoma and endothelial cells that are targeted by Antiangiogenic MEtargidin Peptide AMEP are limited therefore the antiproliferative antimetastatic and antiangiogenic effects of AMEP were investigated in murine melanoma and human endothelial cells after plasmid AMEP gene electrotransfer into the cells in vitro Plasmid AMEP a plasmid coding for the disintegrin domain of metargidin targeting specific integrins had cytotoxic and antiproliferative effects on murine melanoma and human endothelial cells Among the metastatic properties of cells migration invasion and adhesion were investigated Plasmid AMEP strongly affected the migration of murine melanoma and human endothelial cell lines and also affected the invasion of highly metastatic murine melanoma B16F10 and human endothelial cell lines There was no effect on cell adhesion on MatrigelTM or fibronectin in all cell lines The antiangiogenic effect was shown with tube formation assay where human microvascular endothelial cell line HMEC1 proved to be more sensitive to plasmid AMEP gene electrotransfer than the human umbilical vein endothelial cell line HUVEC The study indicates that antiproliferative and antimetastatic biological responses to gene electrotransfer of plasmid AMEP in murine melanoma cells were dependent on the integrin quantity on melanoma cells and not on the expression level of AMEP The strong antiangiogenic effect expressed in human endothelial cell lines was only partly dependent on the quantity of integrins and seemed to be plasmid AMEP dose dependentThe authors acknowledge the financial support from the state budget by the Slovenian Research Agency program no P30003 project no J34211 The research was conducted in the scope of LEA EBAM French–Slovenian European Associated Laboratory Pulsed Electric Fields Applications in Biology and Medicine and COST Action TD1104 We would like to thank Mira Lavric Institute of Oncology Ljubljana Ljubljana Slovenia for all the valuable work she contributed to this research Céline Bouquet is a stockholder and an employee at BioAlliance Pharma The other authors declare no conflict of interest

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  1. The Chemical Nature of the Polar Functional Group of Oxidized Acyl Chain Uniquely Modifies the Physicochemical Properties of Oxidized Phospholipid-Containing Lipid Particles
  2. Gating-Related Molecular Motions in the Extracellular Domain of the I Ks Channel: Implications for I Ks Channelopathy
  3. Effects of Phospholipase A 2 Inhibitors on Bilayer Lipid Membranes
  4. The Effect of Millisecond Pulsed Electric Fields (msPEF) on Intracellular Drug Transport with Negatively Charged Large Nanocarriers Made of Solid Lipid Nanoparticles (SLN): In Vitro Study
  5. Evolution of the Oligopeptide Transporter Family
  6. Slow Gating of Gap Junction Channels and Calmodulin
  7. Molecular Dynamic Simulation of Transmembrane Pore Growth
  8. Genistein Modifies the Activation Kinetics and Magnitude of Phosphorylated Wild-Type and G551D-CFTR Chloride Currents
  9. A Multi-label Classifier for Prediction Membrane Protein Functional Types in Animal
  10. Extracellular Adenine Nucleotides Regulate Na+/H+ Exchanger NHE3 Activity in A6-NHE3 Transfectants by a cAMP/PKA-dependent Mechanism
  11. Part-2: Analytical Expressions of Concentrations of Glucose, Oxygen, and Gluconic Acid in a Composite Membrane for Closed-Loop Insulin Delivery for the Non-steady State Conditions
  12. Prediction of Protein–Protein Interaction Sites with Machine-Learning-Based Data-Cleaning and Post-Filtering Procedures
  13. Salinity-Induced Noise in Membrane Potential of Characeae Chara australis : Effect of Exogenous Melatonin
  14. Swelling-Induced Ca 2+ Influx and K + Efflux in American Alligator Erythrocytes
  15. Tubular Fluid Secretion in the Seminiferous Epithelium: Ion Transporters and Aquaporins in Sertoli Cells
  16. Ionic Currents of Human Trabecular Meshwork Cells from Control and Glaucoma Subjects
  17. Inactivation of Pseudomonas putida by Pulsed Electric Field Treatment: A Study on the Correlation of Treatment Parameters and Inactivation Efficiency in the Short-Pulse Range
  18. Effect of Chloride Channel Inhibitors on Cytosolic Ca 2+ Levels and Ca 2+ -Activated K + (Gardos) Channel Activity in Human Red Blood Cells
  19. Carbon Nanotube-Encapsulated Drug Penetration Through the Cell Membrane: An Investigation Based on Steered Molecular Dynamics Simulation
  20. Influence of Pathogenic Mutations on the Energetics of Translocon-Mediated Bilayer Integration of Transmembrane Helices
  21. Inhibition of Glial Na + and K + Currents by Tamoxifen
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