Authors: ShuJi Ye Yi Luo
Publish Date: 2014/11/05
Volume: 57, Issue: 12, Pages: 1646-1661
Abstract
Sum frequency generation vibrational spectroscopy SFGVS has been demonstrated to be a powerful technique to study the interfacial structures and interactions of biomolecules at the molecular level Yet most previous studies mainly collected the SFG spectra in the frequency range of 1500–4000 cm−1 which is not always sufficient to describe the detailed interactions at surface and interface Thorough knowledge of the complex biophysicochemical interactions between biomolecules and surface requires new ideas and advanced experimental methods for collecting SFG vibrational spectra We introduced some advanced methods recently exploited by our group and others including 1 detection of vibration modes in the fingerprint region 2 combination of chiral and achiral polarization measurements 3 SFG coupled with surface plasmon polaritons SPPs 4 imaging and microscopy approaches and 5 ultrafast timeresolved SFG measurements The technique that we integrated with these advanced methods may help to give a detailed and highspatialresolution 3D picture of interfacial biomolecules
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