Authors:
Publish Date: 1991/03/01
Volume: 5, Issue: 3, Pages: 155-160
Abstract
The longstanding release of locally cytotoxic glutaraldehydeconcentrations from fixed biological materials is one reason for the lackof spontaneous endothelialization in vivo and in vitro of biological graftsand valves Preliminary studies have shown that bovine pericardiumpostfixationtreated with aminoacids may be endothelialized in vitro Totest whether such treatment improves spontaneous endothelialization in vivo8 cm long segments grafts 6 mm ID were interposed into the carotidarteries of 10 sheep Ten grafts were sewn from postfixation treatedpericardium PTP 4 from standard glutaraldehyde fixed pericardium GAPand 6 polytetrafluoroethylane PTFE grafts were implanted to study thedegree of spontaneous endothelialization In two pregnant animals allgrafts occluded 2 PTP 1 GAP 1 PTFE In the remaining animals 1additional PTFE graft occluded and 2 PTFE and 1 GA grafts showedsignificant thrombotic obstruction The patent grafts were harvested at 3months and the surface covered with red thrombus was determined byplanimetry PTP grafts had significantly less 122 +/ 15 thromboticappositions than glutaraldehyde treated grafts 49 +/ 20 P = 001 andPTFE grafts 405 +/ 13 P = 001 In the central areas of the PTPgrafts endothelial cells spread directly on the collagenous matrix andproduced a basal lamina In GAP endothelial cells spread on amorphousmaterial or macrophages and in PTFE grafts only a neointima composed ofmyofibroblasts was endothelialized Neutralization of glutaraldehydeconcentrations by postfixation with aminoacids improves spontaneousendothelialization in vivo in biological materials
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