Authors: Bavita Asthir Amrinder Kaur Amarjit S Basra
Publish Date: 1998/09/01
Volume: 20, Issue: 3, Pages: 285-
Abstract
Soluble acid and neutral invertases purified from the midmilky stage grains of a heatsusceptible HD 2329 and a heattolerant TDR195 genotype of wheat Triticum aestivum L showed marked differences in pH optima Km and Vmax values Both the invertases were more thermostable in TDR195 because of inherent heattolerance ability of this genotype Inhibition of enzyme activity by iodine and its regeneration by βmercaptoethanol implied the involvement of sulphydryl groups for enzyme action Inhibitory effect of sulphydryl group modifiers and various metal ions was much more pronounced in the susceptible genotype than in the tolerant genotype Overall it appears that the observed differences in thermal stability and kinetic behaviour of invertases may be connected to the differential heat sensitivity of the two genotypes studied
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