Journal Title
Title of Journal: Acta Physiol Plant
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Abbravation: Acta Physiologiae Plantarum
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Publisher
Springer-Verlag
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Authors: Qiaomei Ru Xin Wang Tingwu Liu Hailei Zheng
Publish Date: 2012/08/14
Volume: 35, Issue: 1, Pages: 271-282
Abstract
Galanthamine is used as a strong reversible inhibitor of cholinesterase to increase acetylcholine sensitivity Here we sought to determine the effect of nitrogen N application on physiological and proteomic properties of Lycoris aurea L aurea in order to explore the factors that influence galanthamine biosynthesis A marked increase in plant growth was observed under absent nitrogen condition 0 mmol L−1 N while a clear stunting of growth was seen under high nitrogen condition 357 mmol L−1 N Levels of photosynthetic pigments also showed a preference for the absent nitrogen condition The leaves and roots of absent nitrogen plants had significantly higher galanthamine content and lower contents of free amino acids and proline compared with those of control 075 mmol L−1 N On the other hand galanthamine content in L aurea was significantly reduced with high nitrogen treatment However the contents of free amino acids and proline were increased with high nitrogen application suggesting that these play important roles in the storage of nitrogen in L aurea Next we carried out proteomic analysis of the leaves bulbs and roots of L aurea treated with nitrogen by twodimensional gel electrophoresis Using comparative proteomic strategies we found 11 proteins that showed significant differences among the three groups These proteins are involved in a variety of cellular processes including energy metabolism scavenging oxygen free radicals cytoskeleton stabilization galanthamine biosynthesis and cellular homeostasis Out of the 11 proteins the level of phenylalanine ammonialyase was found to correlate with that of galanthamine biosynthesisThis work was supported by the National Natural Science Foundation of China NSFC No 30930076 30770192 30670317 the Foundation of the Chinese Ministry of Education and Program for New Century Excellent Talents in Xiamen University NCETXMU No X07115 to HL Zheng and the Changjiang Scholarship to ZM Pei
Keywords:
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