Authors: Poornananda Madhava Naik Shirugumbi Hanamanthagouda Manohar Hosakatte Niranjana Murthy
Publish Date: 2010/12/24
Volume: 33, Issue: 4, Pages: 1553-1557
Abstract
The present work deals with optimization of adventitious shoot culture of Bacopa monnieri for the production of biomass and bacoside A and has investigated the effects of macro elements NH4NO3 KNO3 CaCl2 MgSO4 and KH2PO4 and nitrogen source NH4 +/NO3 − of Murashige and Skoog Physiol Plant 15473–497 1962 medium MS on accumulation of biomass and bacoside A content Optimum number of adventitious shoots 9933 shoots explant−1 fresh weight 1841 g and dry weight 0150 g were obtained in the medium with 20× strength of NH4NO3 The highest production of bacoside A content was also recorded in the medium of 20× NH4NO3 which produced 17935 mg g−1 DW The number of adventitious shoot biomass and bacoside A content were optimum when the NO3 − concentration was higher than that of NH4 + Maximum number of shoots 7000 shoots explant−1 biomass fresh weight 1137 g and dry weight 0080 g and also bacoside A content 27106 mg g−1 DW were obtained at NH4 +/NO3 − ratio of 1438/3760 mM Overall MS medium supplemented with 20× NH4NO3 is recommended for most efficient bacoside A production
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