Authors: Ganeshan Sivanandhan Manoharan Rajesh Muthukrishnan Arun Murugaraj Jeyaraj Gnanajothi Kapil Dev Annavi Arjunan Markandan Manickavasagam Manickam Muthuselvam Natesan Selvaraj Andy Ganapathi
Publish Date: 2012/10/02
Volume: 35, Issue: 3, Pages: 715-728
Abstract
The influence of cytokinins and culture conditions including medium volume harvest time and elicitation with abiotic elicitors SA/MeJ have been studied for the optimal production of biomass and withanolides in the multiple shoot culture of Withania somnifera Elicitation of shoot inoculum mass 2 g l−l FW with SA at 100 μM in the presence of 06 mg l−l BA and 20 mg l−l spermidine for 4 h exposure time at the 4th week in 20 ml liquid medium recorded higher withanolides production withanolides A 848 mg g−l DW withanolides B 1547 mg g−l DW withaferin A 2955 mg g−l DW and withanone 2344 mg g−l DW which were 114 to 118fold higher than elicitation with MeJ at 100 μM after 5 weeks of culture SAelicited cultures did not exhibit much variation in biomass accumulation when compared to control This cytokinin induces and SAelicited multiple shoot culture protocol provides a potential alternative for the optimal production of biomass and withanolides utilizing liquid culture
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