Journal Title
Title of Journal: Acta Physiol Plant
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Abbravation: Acta Physiologiae Plantarum
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Publisher
Springer Berlin Heidelberg
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Authors: Najmeh Jafari Rofina Yasmin Othman Boon Chin Tan Norzulaani Khalid
Publish Date: 2015/02/17
Volume: 37, Issue: 3, Pages: 45-
Abstract
The present study established a protocol for generating embryogenic cell suspension ECS cultures from an early stage embryogenic callus of Musa acuminata cv ‘Berangan’ The necessary baseline data for morphohistological recognition and the selected gene expression for differentiating embryogenic and nonembryogenic cells were defined to enable early selection as well as for quality assessment which could aid in scaling up the production of these cells In this study male budderived callus on Murashige and Skoog MS medium containing 23 μM 24dichlorophenoxyacetic acid 24D was transferred to M1 medium containing different concentrations of 24D and picloram for somatic embryo initiation ECSs established in liquid MS medium M2a containing 41 μM biotin 10 mg/l ascorbic acid 100 mg/l glutamine 100 mg/l malt extract 45 μM 24D 10 μM zeatin and 20 g/l sucrose were used to study embryogenic development The frequency of embryo development and maturation was influenced by the culture media The highest frequency of embryo development 964 and maturation 664 was achieved on solid MS hormonefree medium Almost 69 of embryos were induced to form shoots on the MS medium containing 34 μM 6benzylaminopurine BAP Histological study indicated that only embryos with distinct layer of protoderm having cells with prominent nucleus and dense cytoplasm were able to regenerate into plantlets Endochitinase and phenylalanine ammonialyase PAL genes were expressed at all the developmental stages and their expression level was tightly regulated during embryo development and reached its maximum in germinating embryos Expression of these genes in embryogenic cells was higher than in nonembryogenic cells The study findings suggested that these genes play an important role in the developmental stage and regeneration pathway of somatic embryos of bananaThis research was supported by Ministry of Science Technology and Innovation Grant 100103PB004 University Malaya Research Grant RP005A13BIO and High Impact Research Grant UMC/625/1/HIR/MOHE/SCI/18 The first author acknowledges the University of Malaya for the award of student fellowship
Keywords:
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- Alleviation of salt stress by plant growth regulators and IAA producing bacteria in wheat
- Characterization of a maize ERF gene, ZmERF1, in hormone and stress responses
- Characterization of a maize ERF gene, ZmERF1, in hormone and stress responses
- Identification for soybean host factors interacting with P3N-PIPO protein of Soybean mosaic virus
- The effects of l -DOPA on root growth, lignification and enzyme activity in soybean seedlings
- Picrorhiza kurrooa : current status and tissue culture mediated biotechnological interventions
- Effect of UV-B radiation on the growth and antioxidant enzymes of Antarctic sea ice microalgae Chlamydomonas sp. ICE-L
- Molecular characterization of a cysteine proteinase inhibitor, PgCPI , from Panax ginseng C. A. Meyer
- Main centers of nitrate and nitrite reduction in young and nodulated yellow lupine ( Lupinus luteus )
- Iridoid and phenylethanoid glycoside production in multiple shoots and regenerated Rehmannia elata N.E. Brown ex Prain plants following micropropagation
- Exogenous 7,8-dihydro-8α-20-hydroxyecdysone application improves antioxidative enzyme system, photosynthesis, and yield in rice under high-temperature condition
- Differential responses of oat cultivars to phosphate deprivation: plant growth and acid phosphatase activities
- Principles of classification of medicinal plants as hyperaccumulators or excluders
- Synergistic roles of leaf boron and calcium during the growing season in affecting sugar and starch accumulation in ripening apple fruit
- Acclimation of clusterbean cotyledon to UV-B radiation in the presence of UV-A: partial restoration of photosynthetic energy balance and redox homeostasis
- Physiological and comparative proteomic analyses in response to nitrogen application in an Amaryllidaceae plant, Lycoris aurea
- Evaluation of clonal integrity in desert date tree ( Balanites aegyptiaca Del.) by inter-simple sequence repeat marker assay
- Responses to excess iron in sweet potato: impacts on growth, enzyme activities, mineral concentrations, and anatomy
- Physiological and molecular response of annual Medicago species to juglone
- Nitric oxide delays chlorophyll degradation and enhances antioxidant activity in banana fruits after cold storage
- Kinetic behaviour of soluble invertases may be connected with the differential heat sensitivity of two wheat genotypes
- Rapid plant regeneration and analysis of genetic fidelity in micropropagated plants of Vitex trifolia : an important medicinal plant
- Impact of osmotic stress on physiological and biochemical characteristics in drought-susceptible and drought-resistant wheat genotypes
- In silico search for drought-responsive genes in plants on the basis of scientific data: case study on poplar roots
- Senescence regulation by alcohols in cut flowers of Calendula officinalis L.
- Ultrastructural studies of plasmodesmatal and vascular translocation of tobacco rattle virus (TRV) in tobacco and potato
- Effects of sucrose concentration and exogenous hormones on growth and periplocin accumulation in adventitious roots of Periploca sepium Bunge
- Effects of macro elements and nitrogen source on biomass accumulation and bacoside A production from adventitious shoot cultures of Bacopa monnieri (L.)
- Effect of culture conditions, cytokinins, methyl jasmonate and salicylic acid on the biomass accumulation and production of withanolides in multiple shoot culture of Withania somnifera (L.) Dunal using liquid culture
- Effects of root colonization by symbiotic arbuscular mycorrhizal fungi on the yield of pharmacologically active compounds in Angelica archangelica L.
- Cadmium elevates level of protein, amino acids and alters activity of proteolytic enzymes in germinating rice seeds
- Transformation of PttKN1 gene to cockscomb
- Effect of arginine and urea on polyamines content and growth of bean under salinity stress
- Exogenous nitric oxide delays salt-induced leaf senescence in cotton ( Gossypium hirsutum L.)
- Morphohistological and molecular profiles during the developmental stages of somatic embryogenesis of Musa acuminata cv . ‘ Berangan’ (AAA)
- Red cabbage extract limits copper stress injury in meristematic cells of Vicia faba
- The effect of phosphorus deficiency on nutrient uptake, nitrogen fixation and photosynthetic rate in mashbean, mungbean and soybean
- The relationship between seedling growth and grain yield under drought conditions in maize and triticale genotypes
- Gene expression profiles in maize ( Zea mays L.) leaves inoculation with southern corn rust ( Puccinia polysora Underw.)
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