Iridoid and phenylethanoid glycoside production in

Authors: Ewelina Piątczak Angelika Talar Łukasz Kuźma Halina Wysokińska

Publish Date: 2015/11/05

Volume: 37, Issue: 12, Pages: 255-

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Abstract

An efficient in vitro plant propagation procedure was established from the shoot tips of Rehmannia elata Shoot proliferation was performed on Murashige and Skoog MS agar medium containing 057 µM of indole3acetic acid IAA and different concentrations 2–8 µM of 6benzylaminopurine BAP kinetin or 2isopentenyladenine 2iP The highest multiplication rate nine shoots and buds per explant after 4 weeks was achieved on MS medium with IAA and 2iP 6 µM All shoots developed an average of 792 roots of 296 mm length after 4 weeks of culture on MS medium with half the macro and micronutrient content 1/2 MS with 057 µM of indole3butyric acid The plantlets were successfully transferred into a greenhouse and then the plants were grown in a field The iridoid and phenylethanoid contents in multiple shoots as well as shoots and roots of 4monthold fieldgrown plants of R elata derived in vitro from shoot tips or from seeds were determined using UHPLC Quantitative differences in production of secondary metabolites were found depending on the type of analyzed plant material Harpagide verbascoside and isoverbascoside were identified in shoot culture whereas additionally catalpol was detected in the shoots and roots of intact plants The highest yield of harpagide was revealed in multiple shoots in the presence of 2iP 4 µM Higher levels of catalpol verbascoside and isoverbascoside were found in in vitro derived plants than in seedraised plants The obtained shoot cultures as well as regenerated R elata plants may be an efficient source of biologically active iridoid catalpol harpagide and phenylethanoid verbascoside isoverbascoside glycosides

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