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Title of Journal: Acta Physiol Plant

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Abbravation: Acta Physiologiae Plantarum

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Springer Berlin Heidelberg

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DOI

10.1002/bimj.4710330711

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1861-1664

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Evaluation of clonal integrity in desert date tree

Authors: Ankita Varshney M Anis
Publish Date: 2013/05/21
Volume: 35, Issue: 8, Pages: 2559-2565
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Abstract

Axillary shoot bud multiplication as a safest mode of micropropagation to obtain clonal progeny was revealed through the application of molecular marker technique in Balanites aegyptiaca Intersimple sequence repeat ISSR markers were used to evaluate the genetic constancy of micropropagated plantlets chosen from a clonal collection of shoots that originated from mature nodal explants mother plant Out of 20 ISSR primers screened ten primers yielded reliable and reproducible patterns of amplified products in all the tested plants In this study on an average 117 bands were amplified per primer A total of 117 bands were scored for the tissue cultureraised plantlets 115 amplification products were monomorphic and 2 bands were polymorphic Based on the ISSR band data 982  genetic uniformity was detected among the regenerants Thus the amplification products validated that the plantlets were truetotype in morphological or growth characteristics when compared with the mother plantAuthors gratefully acknowledge the Department of Science and Technology and the University Grant Commission Govt of India New Delhi for providing research support under DSTFIST 2005 and UGCSAP DRSI 2009 Programs respectively Authors are thankful to fellows Dr S Arif Wali Dr Shashi Bhushan Dr Madan Singh Negi and Mr Shyam Sundar Sharma SRFCSIR from TERI New Delhi India for their indispensable help in executing the experiment


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