Authors: F Espinoza L D Daurelio S C Pessino E M Valle C L Quarin
Publish Date: 2006/04/11
Volume: 258, Issue: 3-4, Pages: 147-159
Abstract
Paspalum notatum Flügge is a waRMSEason forage grass with sexual diploid and apomictic tetraploid races Genetic improvement was achieved in outbreeding diploids The acquisition of artificial sexual tetraploids has raised the possibility of performing crosses and plant improvement at the tetraploid level The objective of our study was to obtain a genetic and cytoembryological characterization of a germplasm collection of P notatum including 31 accessions from seven countries of America and 11 experimentally obtained genotypes Morphology of mature gametophytes was observed to assess the mode of reproduction of the accessions A total of 1342 AFLP fragments were generated across the 42 genotypes and from two reference taxa P urvillei and P procurrens AFLP data were converted into a binary matrix and similarity relationships were established The genetic distance among all the accessions showed a maximum value of 036 In addition eleven AFLP fragments were observed exclusively in apomictic plants which could be linked to genomic regions implicated in the control of apospory
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