Authors: Andrei Caíque Pires Nunes Ester Ujiie Nogueira Andreia Barcelos Passos Lima Gontijo Carlos Roberto Carvalho Wellington Ronildo Clarindo
Publish Date: 2013/03/16
Volume: 299, Issue: 6, Pages: 1135-1140
Abstract
The Bromeliaceae family has been traditionally distributed in the subfamilies Bromelioideae Tillandsioideae and Pitcairnioideae However phylogenetic studies have provided other classifications highlighting the need for analyses in order to characterize the genome of different species from this family In this sense the present work aimed to determine nuclear 2Cvalue and base composition characterize the chromosomes and establish the karyogram of Pitcairnia flammea Flow cytometry yielded 2C = 144 pg AT = 6428 and GC = 3572 for this species indicating its relatively small genome size Despite reduced length and morphological similarity of the chromosomes P flammea metaphases presented wellspread chromosomes with welldefined primary constriction without chromatin damage and cytoplasmic background These aspects allowed morphometric chromosomal characterization and assembly of the first karyogram of a Bromeliaceae species The karyogram displayed 2n = 50 chromosomes of which all were submetacentric Karyomorphological analysis revealed grouped pairs of cytogenetically identical chromosomes 2–3 4–5 6–9 10–17 18–19 20–23 and 24–25 plus one isolated chromosome 1 not identical to any other This result suggests an allopolyploid origin for the P flammea genome Thus the present investigation contributed with karyotype data for taxonomic and evolutionary aspects of this groupThe authors are grateful to Conselho Nacional de Desenvolvimento Científico e Tecnológico CNPq Brasília DF Brazil Fundação de Amparo à Pesquisa do Espírito Santo FAPES Vitória ES Brazil Fundação de Amparo à Pesquisa do Estado de Minas Gerais FAPEMIG Belo Horizonte MG Brazil and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior CAPES Brasília DF Brazil for financial support
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