Evolution of mitochondrial gene content loss of g

Authors: Binbin Lei Shuangshuang Li Guozheng Liu Zhiwen Chen Aiguo Su Pengbo Li Zhaohu Li Jinping Hua

Publish Date: 2013/06/04

Volume: 299, Issue: 10, Pages: 1889-1897

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Abstract

Mitochondrion is a kind of cell organelle known as the engine house of the cells in the performance of the production of energy in the form of ATP and the regulation of cellular metabolism in programmed cell death Plant mitochondria are involved in the formation of cytoplasm male sterility and the mechanism of restoration Its genomes offer useful information in analysis of the evolution dynamics The mitogenomes mitochondrial genomes of 2074A a cytoplasmic male sterile line of Gossypium harknessii cytoplasm was sequenced by Solexa strategy and assembled by SOAP de novo Combined with public data the sequences of nine mitochondrial functional genes in 20 taxa were used to reconstruct phylogenetic trees and further to demonstrate the variations of mitogenomes in higher plants The sequence size genome composition and the number of genes varied in mitogenomes while the genes related to oxidative respiratory chain remain conserved In examined mitogenomes the number of proteincoding genes of higher plants varied from 24 to 42 And gene conservatism was quite different Gene gain or loss entirely existed widely genes insertion and loss of intron s and some altered as pseudogenes were checked loss of tRNAs and insertion of cpDNA transferring happened frequently and syntenic gene clusters were found More than 50  of intergenic regions were mainly accumulated by repeats and noncoding sequences The variable mitogenomes existed conservatism but it demonstrated that the linear relationship was not parallel to that in mitogenomes of different species in evolution The mitogenome of 2074A harbored 56 functional genes and changed quite a lot in sequences while there were a few linear gene clusters and conserved flanking sequences of functional genes Generally the information was helpful for understanding the results in mitogenome evolutionThis work was supported in part by the National Natural Science Foundation of China Grant No 31171591 and the Key Project of Chinese Ministry of Education Grant No 107012 and Training Program Foundation for the New Century Excellent Talents in University NCET060106 to J HUA Thanks to Prof Dr ShuMiaw CHAW Biodiversity Research Center Academia Sinica Taipei China and Dr Yi HUANG Oil Crops Research Institute Chinese Academy of Agricultural Sciences for their helpful discussion

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