Authors: XiaoRong Wang HaoRu Tang HanWu Zhang BiFeng Zhong WuFeng Xia Yuan Liu
Publish Date: 2009/09/16
Volume: 283, Issue: 1-2, Pages: 9-
Abstract
Twelve taxa belonging to two subsections of section Idaeobatus in Rubus L from southwestern China were characterized by karyotypic palynological and random amplified polymorphic DNA RAPD data as follows 1 The 12 taxa were all diploid species 2n = 2x = 14 among which the chromosome counts for R mesogaeus var oxycomus R subtibetanus R ellipticus var obcordatus R inopertus var echinocalyx and R stans were reported for the first time 2 All taxa except for R ellipticus and R pinfaensis could be distinguished from each other by karyotype pollen morphology and RAPD markers Karyotypes were mainly characterized by the difference in numbers and positions of submetacentric chromosomes and chromosomes with satellited pair the index of the karyotypic asymmetry and the ratio of the longest to the shortest chromosome Pollen morphology were mainly characterized by the discrepancy in specific pollen size P/E ratio colpi width distance between the apices of two ectocolpi and exine ornamentation characters The cluster results based on RAPD markers were consistent with morphology classification except for R pinfaensis 3 Based on the general data of karyotypic palynological and RAPD R ellipticus var obcordatus should be treated as a species R obcordatus R ellipticus and R pinfaensis should be combined as R ellipticus and it was more reasonable to place the combinants and R obcordatus into subsection Stimulantes rather than into subsection PungentesThe authors are thankful to the Program for the National Natural Science Foundation of China 30671454 and the Education Bureau of Sichuan Province China for financial support 2004A025 We particularly thank Prof Guanghui Yang Sichuan Agricultural University for her help in the course of specimen identification
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