Authors: T Brune M Thiv K Haas
Publish Date: 2008/05/27
Volume: 274, Issue: 1-2, Pages: 67-
Abstract
The applicability of “intersimple sequence repeats” ISSRPCR as a molecular character complex for differentiation of Equisetum taxa is described with a special focus on the detection of hybrids All Central European Equisetum species and the four most frequent hybrids are considered E arvense E fluviatile E telmateia E palustre E sylvaticum E pratense E × litorale E ramosissimum E hyemale E variegatum E × moorei E × trachyodon and E × meridionale The ISSR banding patterns are highly typical for each species The positions of the clusters of hybrid taxa prove their hybrid origin and enable identification of the parental species Genetic similarities of populations suggest that in some species vegetative reproduction predominates eg E arvense whereas for others eg E telmateia sexual reproduction seems to be more frequent In addition to the molecular analyses the characteristics of stem cross sections of the four hybrids and their parental species are shown and discussedWe are grateful to R Böcker B Schäfer and A SchmidLebuhn for providing plant material and information on sampling sites We thank E Kandeler for support with additional analysis opportunities For technical assistance and various help we thank M Heklau T Jossberger and R Zipper
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