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Title of Journal: Plant Syst Evol

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Abbravation: Plant Systematics and Evolution

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Springer-Verlag

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DOI

10.1002/vipr.201000409

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1615-6110

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Mitotic evidence for the tetraploid nature of Emp

Authors: W R Clarindo C R de Carvalho B M G Alves
Publish Date: 2007/03/22
Volume: 265, Issue: 1-2, Pages: 101-107
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Abstract

The high number very small size and morphological similarity of the chromosomes and low metaphasic indexes obtained in root meristems have hindered the progress in cytogenetic and evolutionary studies of Glycine max In order to contribute to the solving of these problems we have developed a method based on the use of DNA synthesis inhibiting and antimicrotubule solutions and enzymatic maceration and airdrying techniques Besides we have employed a digital image analysis system tool This method provided prometaphasic and metaphasic chromosomes showing welldefined primary and secondary constrictions which facilitated the pairing of homologues and assembly of the first karyogram for G max This species possesses twenty chromosome pairs being six metacentric and fourteen submetacentric The karyograms support its tetraploid nature 4x = 40 specifically for the presence of chromosomes with identical morphology and suggest that chromosome rearrangements may have occurred during the speciation of G max

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