Authors: Yulia Burtseva Natalia Verigina Victoria Sova Mikhail Pivkin Tatiana Zvyagintseva
Publish Date: 2006/06/10
Volume: 18, Issue: 3-5, Pages: 375-
Abstract
Marine filamentous fungi 103 strains isolated from various marine habitats were studied for their ability to produce extracellular Oglycosylhydrolases Cultural filtrates of these strains were shown to contain a series of glycanases laminarinases amylases cellulases pustulanases and glycosidases βglucosidases Nacetylβglucosaminidases βgalactosidases αmannosidasesTwo species of marine fungi from different habitats were chosen for isolation of laminarinases and detailed study on enzyme properties The fungus Chaetomium indicum associated with the alga Fucus evanescens C Agardh was collected near the Kuril Islands and T aureviride was sampled from bottom deposits of South China Sea Properties of extracellular laminarinases were similar temperature optimums 40–45 ∘C molecular masses 54–56 kDa K m 01–03 mg ml−1 Temperature stability of laminarinase of C indicum was significantly higher than those from T aureveride It is shown that these enzymes are specific to β13bonds in glucans release predominantly glucose from laminaran and do not catalyze reaction of transglycosylation Accoding to these data enzymes are exo13βDglucanglucanohydrolases EC 32158 Inhibitor analysis demonstrated the significant role of tryptophan and tyrosine residues in the catalytic activity of enzymes Molecules of T aureviride laminarinase contained the functionally important thiol group
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