Total phenolic content and biological activities o

Authors: Maya Puspita Maureen Déniel Ita Widowati Ocky Karna Radjasa Philippe Douzenel Christel Marty Laurent Vandanjon Gilles Bedoux Nathalie Bourgougnon

Publish Date: 2017/03/11

Volume: 29, Issue: 5, Pages: 2521-2537

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Abstract

Seaweeds are potentially excellent sources of bioactive metabolites that could represent useful leads in the development of new functional ingredients in pharmaceutical and cosmetic industries In the last decade new marine bioprocess technologies have allowed the isolation of substances with biological properties The brown alga Sargassum muticum Yendo Fensholt Ochrophyta was enzymatically hydrolyzed to prepare watersoluble extracts by using six different commercially available carbohydratedegrading enzymes and two proteases Evaluation of 22diphenyl1picrylhydrazyl DPPH and ferric reducing antioxidant power FRAP antioxidant tyrosinase elastase and biofilm inhibition antibacterial and antiviral activities as well as evaluation of cytotoxicity were realized for each extract Total phenolic content was measured for extract characterization and solidphase extraction was useful to purify the enzymatic extract Soluble total phenolic content of S muticum Viscozyme extract was highest with 64 of dry weight Enzymatic Celluclast and Viscozyme extracts had the lowest value of DPPH IC50 indicating a strong antiradical activity 06 mg mL−1 in comparison with other enzymes The ferric reducing antioxidant power ranged between 487 μM Fe2+ Eq digested with Viscozyme and 608 μM Fe2+ Eq digested with Amyloglucosidase Tyrosinase inhibition activity of S muticum Neutrase extract was 413 higher compared to other enzymes Elastase inhibition activity of S muticum Shearzyme extract had highest activity 328 All enzymatic extracts showed no cytotoxic effect towards the kidney Vero cells Meanwhile only S muticum Neutrase and Alcalase extracts exhibited potential antiviral activity In addition S muticum Viscozyme and Shearzyme extracts showed promising activity in suppressing the biofilm formation against Pseudomonas aeruginosa and Escherichia coli respectively Purification of S muticum Viscozyme extracts by solidphase extraction managed to concentrate the phenolic content and improve the bioactivity These results indicate the promising potential of enzymeassisted followed by solidphase extraction in recovering phenolic content and in improving its bioactivityThis study was a collaboration research between Université Bretagne Sud UBS Vannes France and Diponegoro University UNDIP Semarang Indonesia under DoubleDegree programme supported by the Directorate of Higher Education DIKTI The authors would like to thank all members of the Laboratoire de Biotechnologie et Chimie Marines LBCM who had been very helpful and supportive during the research Special thanks are due to Dr Valérie StigerPouvreau from LEMAR Université de Bretagne Occidentale UBO for her help in identifying the sample Dr Nolwenn Terme from UBS for her corrections and Dr Isuru Wijesekara for his interesting ideas and support during the purification

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