Authors: Fatemeh Rahimi Mehdi Tabarsa Masoud Rezaei
Publish Date: 2016/03/07
Volume: 28, Issue: 5, Pages: 2979-2990
Abstract
Response surface methodology was employed to optimize the ultrasoundassisted extraction of crude polysaccharides from the cell wall of Ulva intestinalis The impacts of four independent variables including extraction temperature X 1 50–90 °C extraction time X 2 20–40 min the ratio of water to raw material X 3 50–70 and pH X 4 7–9 were investigated through Box–Behnken experimental design The experimental data were adequately fitted into a secondorder polynomial model and optimized conditions were as follows extraction temperature 66 °C extraction time 40 min water to raw material ratio 50 and pH 70 Fourier transform infrared FTIR results revealed the presence of sulfate esters indicated by the peaks at 850 and 1256 cm−1 derived from the bending vibration of C–O–S and stretching vibration of S–O of sulfate The experimental yield achieved under these conditions was 830 which was well in close agreement with the value 836 predicted by the response surface methodology RSM model The extracted polysaccharides exhibited appreciable 22diphenylpicrylhydrazyl DPPH radical scavenging and reducing power as well as macrophagestimulating capacity indicating their potential value for health and food industry
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