Authors: Emer Shannon Nissreen AbuGhannam
Publish Date: 2016/11/02
Volume: 29, Issue: 2, Pages: 1027-1036
Abstract
Fucoxanthin is a xanthophyll pigment which occurs in marine brown algae Phaeophyceae The antidiabetic antiobesity anticancer and antioxidant properties of fucoxanthin have been widely reported Macroalgae particularly brown seaweeds grow prolifically around Irish coasts representing a valuable resource of nutraceuticals such as fucoxanthin for functional food applications The aim of this study was to maximise the solvent extraction yield from three anatomically discrete regions of the seaweed thallus blade stipe and holdfast Response surface methodology was applied to determine optimum parameters for extraction of fucoxanthin from the seaweed Fucus vesiculosus as a model species A central composite design was applied with four extraction variables time 30–70 min temperature 30–70 °C solvent pH 50–90 and percentage acetone 30–70 Fucoxanthin content of extracts was quantified by highperformance liquid chromatography Percentage acetone was found to have the most significant P = 00002 effect on fucoxanthin yield followed by pH P = 0028 and temperature P = 0049 Multiple response optimisation determined that fucoxanthin yield from F vesiculosus may be maximised by incubating at 300 °C for 365 min pH 57 with 622 acetone Optimised responses were applied to a further nine brown seaweeds Alaria esculenta Ascophyllum nodosum Fucus serratus Himanthalia elongata Laminaria digitata Laminaria hyperborea Pelvetia canaliculata Saccharina latissima and Saccorhiza polyschides In all species the blades contained significantly more fucoxanthin than stipes while holdfasts contained the least Alaria esculenta blade had the greatest yield 0870 mg g−1 dry mass followed by F vesiculosus blade 0699 mg g−1 and L digitata blade 0650 mg g−1
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