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Title of Journal: Euphytica

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Abbravation: Euphytica

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Springer Netherlands

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DOI

10.1007/bf00288183

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1573-5060

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Simple Sequence Repeat Markers for Detecting Sourc

Authors: Anna V Blenda Gregory L Reighard William V Baird Albert G Abbott
Publish Date: 2006/03/01
Volume: 147, Issue: 3, Pages: 287-295
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Abstract

Peach tree short life PTSL is a severe and complex disease syndrome in the Southeast of the United States that causes significant damage to peach orchards and affects more than 70 of the peach acreage in the region The ring nematode is known to be one of the major factors strongly associated with the PTSL syndrome A peach rootstock Guardian® Brand BY5209 also referred to as Guardian® or BY5209 which is tolerant to ring nematode and PTSL was released in 1994 To investigate the genetic relatedness of PTSL tolerant genotypes and identify possible parents of Guardian® selections as sources of the PTSL tolerance trait 98 genotypes derived from the BY5209 pedigree and others showing different degrees of field resistance or susceptibility to PTSL were selected for simple sequence repeat analysis Nineteen of 32 microsatellite loci were polymorphic Allele number averaged 27 per locus with heterozygosity levels ranging from 007 to 060 Cluster analysis of these genotypes produced a dendrogram in agreement with their putative pedigrees Thirtyeight F4 genotypes from Guardian pedigree were used for the parental identification analyses Of those nine most likely parental genotypes were identified for 10 field tested F5 genotypes produced from openpollinated selections of BY5209

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