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Title of Journal: Euphytica

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Abbravation: Euphytica

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Springer Netherlands

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DOI

10.1007/s10483-007-0802-1

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1573-5060

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Interspecific cross of Emphasis Type="Italic"Bra

Authors: Rick A Bennett Mohan R Thiagarajah Jane R King M Habibur Rahman
Publish Date: 2008/08/26
Volume: 164, Issue: 2, Pages: 593-601
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Abstract

Interspecific hybrids were produced from reciprocal crosses between Brassica napus 2n = 38 AACC and B oleracea var alboglabra 2n = 18 CC to introgress the zeroerucic acid alleles from B napus into B oleracea The ovule culture embryo rescue technique was applied for production of F1 plants The effects of silique age as measured by days after pollination DAP and growth condition temperature on the efficiency of this technique was investigated The greatest numbers of hybrids per pollination were produced under 20°/15°C day/night at 16 DAP for B oleracea ♀ × B napus crosses while under 15°/10°C at 14 DAP for B napus ♀ × B oleracea crosses Application of the ovule culture technique also increased the efficiency of BC1 F1 × B oleracea hybrid production by 10fold over in vivo seed set The segregation of erucic acid alleles in the selfpollinated backcross generation ie in BC1S1 seeds revealed that the gametes of the F1 and BC1 plants carrying a greater number of Agenome chromosomes were more viable This resulted in a significantly greater number of intermediate and a smaller number of higherucic acid BC1S1 seedsFunding for this project by Natural Sciences and Engineering Research Council of Canada NSERC and Alberta Canola Producers Commission ACPC to the last author is gratefully acknowledged The authors are also thankful for the technical assistance of An Vo of the Canola Program

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