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Title of Journal: Euphytica

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Abbravation: Euphytica

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Springer Netherlands

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1573-5060

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A genetic map of an interspecific diploid pseudo t

Authors: Germán Ariel López Gartner Susan R McCouch María Del Pilar Moncada
Publish Date: 2013/04/23
Volume: 192, Issue: 2, Pages: 305-323
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Abstract

Coffee is globally one of the most important export crops and is a prominent part of the economy in more than 50 countries in Latin America Africa and Asia In Colombia it has been the leading export commodity for more than a century However genetic research on coffee has been rather sparse and mainly focused on the two major cultivated species Coffea arabica L and C canephora P leaving unexplored the genetic potential in other species In this study an interspecific mapping population consisting of 101 F1 hybrid plants from a cross between the diploid species C liberica and C eugenioides was evaluated for genetic segregation at 618 molecular marker loci Of these 168 SSRs and two ESTs exhibited polymorphic patterns that allowed segregation analysis and genetic linkage estimations A genetic map consisting of 146 codominant loci and 11 predicted linkage groups was constructed using the mapping software JoinMap 30 The conjoined maternal/paternal map length is 79868 cM has an average saturation density of 601 cM/interval and covers an estimated 66–86  of the diploid coffee genome Approximately 24  of loci had null alleles and 235  exhibited segregation distortion Knowledge derived from this study has important applications for quantitative trait locus analysis and markerassisted selection in Colombian coffee breeding programs

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