Authors: Liang Wang Genying Li Xianchun Xia Zhonghu He Peiyuan Mu
Publish Date: 2008/05/16
Volume: 164, Issue: 3, Pages: 745-752
Abstract
Grain hardness plays an important role in determining both milling performance and quality of the enduse products produced from common or bread wheat The objective of this study was to characterize allelic variations at the Pina and Pinb loci in Xinjiang wheat germplasm for further understanding the mechanisms involved in endosperm texture formation and the status of grain texture in Chinese bread wheat A total of 291 wheat cultivars including 56 landraces and 95 introduced and 140 locally improved cultivars grown in Xinjiang were used for SKCS measurement and molecular characterization Among the harvested grain samples 185 636 40 137 and 66 227 were classified as hard mixed and soft respectively Eight different genotypes for the Pina and Pinb loci were identified including seven previously reported genotypes viz PinaD1a/PinbD1a PinaD1a/PinbD1b PinaD1b/PinbD1a PinaD1a/PinbD1p PinaD1a/PinbD1q PinaD1a/PinbD1aa PinaD1a/PinbD1ab and a novel Pinb allele PinbD1ac This new allele detected in Kashibaipi local landrace and Red Star from Russia has a double mutation at the 257th G to A substitution and 382nd C to T substitution nucleotide positions of the coding region PinaD1b PinbD1b and PinbD1p were the most common alleles in Xinjiang wheat germplasm with frequencies of 143 381 and 286 in hard textured landraces 255 569 and 118 in hard introduced cultivars and 248 478 and 265 in hard locally improved cultivars respectively The restriction enzymes ApaI SapI BstXI and SfaNI were used to identify PinbD1ab or PinbD1ac PinbD1b PinbD1e and PinbDg respectively by digesting PCR products of the Pinb gene The unique grain hardness distribution in Xinjiang bread wheat as well as the CAPs markers for identification of the Pinb alleles provided useful information for breeding wheat cultivars with optimum grain texturesThe authors are grateful to Prof Bob McIntosh University of Sydney for reviewing this manuscript This project was funded by the Xinjiang Bingtuan Doctoral Foundation 05JC02 the Taishan Scholarship Program from the Shandong Provincial Government and the National Basic Research Program 2002CB11300
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