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Title of Journal: Euphytica

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Abbravation: Euphytica

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Springer Netherlands

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1573-5060

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Construction of a highdensity SSR markerbased li

Authors: Manli Li Nana Yuyama Mariko Hirata Jianguo Han Yunwen Wang Hongwei Cai
Publish Date: 2009/06/28
Volume: 170, Issue: 3, Pages: 327-338
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Abstract

A consensus genetic linkage map with 447 SSR markers was constructed for zoysiagrass Zoysia japonica Steud using 86 F1 individuals from the cross ‘Muroran 2’ × ‘Tawarayama Kita 1’ The consensus map identified 22 linkage groups and had a total length of 20099 cM with an average map density of 48 cM When compared with a previous AFLPSSR linkage map the SSR markers from each linkage group mapped to similar positions in both maps Eight pairs of linkage groups from the AFLPSSR map were joined into eight new groups in the current map This zoysiagrass consensus map contained 35 SSR markers exhibiting high homology with rice genomic sequences from known chromosomal locations This allowed synteny to be identified between Zoysiagrass linkage groups 2 3 9 19 and rice chromosomes 3 12 2 7 respectively These results provide important comparative genomics information and the new map is now available for quantitative trait locus analysis markerassisted selection and breeding for important traits in zoysiagrassThis study was partially funded by a grant from the Japan Racing Association National Natural Science Foundation of China No 30771372 and National Basic Research Program 973 Program of China 2007CB108902 Ms ML Li is grateful to the China Scholarship Council No 2007 3020 for supporting her stay at the Forage Crop Research Institute of the Japan Grassland Agriculture and Forage Seed Association We also thank Dr Alan V Stewart PGG Wrightson Seeds Lincoln New Zealand and the two anonymous reviewers for their valuable comments on this manuscript

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