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Title of Journal: Eur J Appl Physiol

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Abbravation: European Journal of Applied Physiology

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Springer-Verlag

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1439-6327

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Strenuous exercise increases late outgrowth endoth

Authors: Daniel Thorell Mats Borjesson Pia Larsson Erik Ulfhammer Lena Karlsson Smita DuttaRoy
Publish Date: 2009/08/12
Volume: 107, Issue: 4, Pages: 481-
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Abstract

Endothelial progenitor cells EPCs and late outgrowth endothelial cells OECs seem to play an important role in vessel formation While EPCs seem to exert their function mainly through a paracrine effect the OECs can develop into mature endothelial cells and form tubular structures Exercise is known to increase angiogenic factors that can mobilize EPCs however the effect on OECs is not known We investigated the response to a single session of strenuous exercise on OECs vascular endothelial growth factor VEGF and inflammatory cell levels in the healthy Eleven healthy subjects performed 1 h of spinning exercise Blood samples were collected at 1 6 24 and 48 h postexercise for cell culture and biochemical analysis OEC colonies doubled one hour after the spinning session baseline 45 ± 43 vs 90 ± 37 P  005 Serum VEGF increased from 194 ± 107 pg/ml at baseline to 224 ± 111 pg/ml after 1 h p = ns and neutrophilic granulocytes increased from 373 ± 138 at baseline to 908 ± 105 at 1 h P  001 The increased levels of OECs VEGF and neutrophilic granulocytes declined gradually at the following time points VEGF levels and neutrophilic granulocytes were highly correlated to OEC levels r = 0903 VEGF and r = 085 neutrophilic granulocytes respectively Strenuous physical activity increases OEC colonies and is correlated to serum VEGF and neutrophilic granulocytes levels An acute exerciseinduced inflammatory response might be responsible for the VEGF release and subsequent increase of OECs The clinical importance of these findings remains to be elucidated


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