Authors: Borja Guerra Jesus Gustavo PonceGonzález David MoralesAlamo Amelia GuadalupeGrau Kristian Kiilerich Teresa Fuentes Stine Ringholm Rasmus Sjørup Biensø Alfredo Santana Carsten Lundby Henriette Pilegaard José A L Calbet
Publish Date: 2013/11/29
Volume: 114, Issue: 2, Pages: 345-357
Abstract
Deltoid and vastus lateralis muscle biopsies and blood samples were obtained from 12 healthy young men mean ± SD BMI 228 ± 27 kg/m2 before and after 7 days of bed rest Leptin receptor isoforms OBRs suppressor of cytokine signaling 3 SOCS3 and protein tyrosine phosphatase 1B PTP1B protein expression and signal transducer and activator of transcription 3 STAT3 phosphorylation were analyzed by Western blotAfter bed rest basal insulin concentration was increased by 53 P 005 the homeostasis model assessment HOMA by 40 P 005 and serum leptin concentration by 35 P 005 with no changes in body fat mass Although the soluble isoform of the leptin receptor sOBR remained unchanged the molar excess of leptin over sOBR was increased by 14fold after bed rest P 005 OBRs and SOCS3 protein expression and STAT3 phosphorylation level remained unaffected in deltoid and vastus lateralis by bed rest as PTP1B in the deltoid PTP1B was increased by 90 with bed rest in the vastus lateralis P 005 There was a linear relationship between the increase in vastus lateralis PTP1B and the increase in both basal insulin concentrations r = 066 P 005 and HOMA r = 068 P 005 with bed restOne week of bed rest is associated with increased leptin levels without augmenting STAT3 phosphorylation indicating some degree of leptin resistance in skeletal muscle which can be explained at least in part by an elevation of PTP1B protein content in the vastus lateralis muscle
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