Authors: Jie Hao Zhenming Hu Qixin Zheng Xiaodong Guo Yulin Song
Publish Date: 2009/11/13
Volume: 24, Issue: 5, Pages: 768-
Abstract
K16 and RGDcontaining peptide was used to modify the surface of threedimensional PLGAASPPEG matrix then the modified PLGAASPPEG was incubated in modified simulated body fluid SBF The biomineralization of the modified PLGAASPPEG was explored and the peptide was synthesized with solid phase synthesis technology and linked covalently to PLGAASPPEG through crosslinker SulfoLCSPDP which was characterized with XPS The modified PLGAASPPEG Experiment group EG and PLGAASPPEG Control group CG were all incubated into SBF for 10 d and the growth of hydroxyapatite HA nanocrystals was confirmed with XRD EDS and SEM HPLC shows that peptide purity is 9413 while MS analysis shows that molecular value of peptide is 274126 Binding energy of the sulphur in EG was 164 eV is detected by XPS and the ratio of carbon and sulphur is 9974601014 SEM analysis demonstrates the better growth of bonelike HA nanocrystals in EG than that in CG The component of mineral in EG consisted mainly of hydroxyapatite containing low crystalline nanocrystals and the Ca/P ratio is about 160 which is similar to that of natural bone while the Ca/P ratio in CG is 152 PLGAASPPEG modified with peptide provided enough functional groups for biomineralization and possessed the bonelike structure
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