Authors: Liyun Ding Fang Yuan Lanfen Huang Jun Huang Xiaofang Liu Bing Liang
Publish Date: 2014/08/05
Volume: 29, Issue: 4, Pages: 848-853
Abstract
A novel fluorescent probe 9412diaminebenzeneN1phenylacridine DABPA was synthesized for the detection of nitric oxide NO and characterized by IR 1HNMR and EIMS spectroscopy Based on a photoelectron transfer mechanism the fluorescence intensities of DABPA were investigated with the different concentrations of NO Under the optimal experimental conditions the fluorescence intensity of DABPA had a good linear relationship R 2=09977 with NO concentration in the range from 1×10−7 to 15×10−6 mol/L with a detection limit of 1×10−8 mol/L The cytotoxicity induced by DABPA was evaluated by the MTT 345dimethylthiazol2yl25diphenyl tetrazolium bromide assay for biological application Furthermore the probe DABPA had also been successfully applied to realtime image NO produced in PC12 cells in the presence of LarginineFunded by the National Natural Science Foundation of China Nos 50802069 81100890 51272191 the Fundamental Research Funds for the Central Unversities WUT 2013IV010 and the Students Innovation and Entrepreneurship Training Program of WHUT20141049701012
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