Authors: Xingjian Hu Nianguo Dong Jiawei Shi Huadong Li Cheng Deng Cuifen Lu
Publish Date: 2015/01/31
Volume: 30, Issue: 1, Pages: 193-197
Abstract
To investigate the effects of polyethylene glycol crosslinking on the mechanical properties 80 porcine aortic valves were harvested decellularized and introduced with sulfhydryl Then the valves were randomly assigned into 5 experimental groups and 1 control group n=16 For the valves in those experimental groups branched polyethylene glycol diacrylate PEG of 5 different molecular weights 34 8 12 20 40 kDa were synthesized and crosslinked with them respectively The efficiency of the crosslinking was determined by measuring the amount of residual thiol group and the mechanical properties of the crosslinked valve leaflets were assessed by uniaxial planar tensile testing The efficiency of the PEG 20 kDa group was 7072±233 obviously superior to that of the other groups p005 Tensile test proved that branched PEG crosslinking can significantly enhance the mechanical behaviors of the decellularized valve leaflet and the Young’s modulus of each group was positively correlated with the molecular weight of PEG It was concluded that branched PEG with the molecular weight of 20 kDa can effectively crosslink the decellularized porcine aortic valves and improve their mechanical properties which makes it a promising crosslinker that can be used in the modification of decellularized tissue engineering valves
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