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Title of Journal: Mol Cells

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Abbravation: Molecules and Cells

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Publisher

Korean Society for Molecular and Cellular Biology

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DOI

10.1007/bf00173763

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ISSN

0219-1032

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Identification and molecular characterization of P

Authors: JaeWon Huh DaeSoo Kim HongSeok Ha Kung Ahn KyuTae Chang ByungWook Cho HeuiSoo Kim
Publish Date: 2009/02/05
Volume: 27, Issue: 1, Pages: 119-123
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Abstract

Porcine endogenous retroviruses PERVs gamma1 in the pig genome have the potential to act as harmful factors in xenotransplantation pigtohuman Long terminal repeats LTRs are known to be strong promoter elements that could control the transcription activity of PERV elements and the adjacent functional genes To investigate the transcribed PERV gamma1 LTR elements in pig tissues bioinformatic and experimental approaches were conducted Using RTPCR amplification and sequencing approaches 69 different transcribed LTR elements were identified And 69 LTR elements could be divided into six groups 15 subgroups by internal variation including tandem repeated sequences insertion and deletion INDEL Remarkably all internal variations were indentified in U3 region of LTR elements Taken together the identification and characterization of various PERV LTR transcripts allow us to extend our knowledge of PERV and its transcriptional study

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