Mitochondrial oxidative phosphorylation reserve is

Authors: Min Jeong Ryu Soung Jung Kim Min Jeong Choi Yong Kyung Kim Min Hee Lee Seong Eun Lee Hyo Kyun Chung Saet Byel Jung HyunJin Kim Koon Soon Kim Young Suk Jo Gi Ryang Kweon ChulHo Lee Minho Shong

Publish Date: 2013/02/20

Volume: 35, Issue: 2, Pages: 134-141

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Abstract

Adipocyte differentiation requires the coordinated activities of several nuclear transcription factors Recently mitochondria biogenesis was reported to occur during adipocyte differentiation and following treatment with thiazolidinediones in vitro and in vivo Crif1 is a translational factor for mitochondrial DNA mtDNA and is important for transcription of the mitochondrial oxidative phosphorylation OXPHOS complex To investigate the role of OXPHOS in adipogenesis we analyzed adipocyte differentiation following disruption of Crif1 in vitro and in vivo The adiposespecific Crif1 knockout mouse had a lower body weight and less fat mass than wildtype mice Furthermore adipocytes were smaller and had a dysplastic morphology in the adiposespecific Crif1 knockout mouse 3T3L1 adipocytes or adiposederived stem cells ADSCs that lacked Crif1 expressed lower levels of mtDNAencoded OXPHOS subunits and adipocyte differentiation was disrupted Rosiglitazone treatment did not induce adipogenesis or mitochondria biogenesis in Crif1 knockout ADSCs These results show that mitochondrial OXPHOS and Crif1 are required for rosiglitazone and hormoneinduced adipogenesis

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