Expression and imprinting analysis of AK044800 a

Authors: Zhengbin Han Qi Liu Zhijun Huang Wei Cui Yijun Tian Weili Yan Qiong Wu

Publish Date: 2013/03/18

Volume: 35, Issue: 4, Pages: 285-290

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Abstract

Recent advances of induced pluripotent stem cells iPSCs has demonstrated that full development potential is closely related with the expression state of noncoding RNAs ncRNAs of the Dlk1Dio3 imprinted gene cluster However few of them especially the long noncoding RNAs lncRNAs have been characterized in detail AK044800 is a transcript from the Dlk1Dio3 imprinted region with little known information This study reports original data on the expression pattern of AK044800 during embryogenesis Expression analysis showed that AK044800 was specifically expressed in the brain at midgestation E95 and E115 And at E155 its expression was mainly concentrated in the forebrain In the lategestation stage E185 AK044800 expression was weaker in the brain and began to emerge in some other tissues during this period Notably the expression of AK044800 was biallelic in the brain unlike other noncoding transcripts from this imprinted region In addition its expression was dependent on inbred mouse strains This may be the first lncRNA that has been identified with a different expression between inbred mouse strains This study may provide useful clues for further investigations of expression regulation and functions of lncRNAs of the Dlk1Dio3 imprinted region

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