Selection and characterization of tenascin C targe

Authors: Mee Young Kim Ok Ran Kim Yong Seok Choi Heuiran Lee Keerang Park ChoonTaek Lee Keon Wook Kang Sunjoo Jeong

Publish Date: 2011/12/01

Volume: 33, Issue: 1, Pages: 71-77

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Abstract

Since tenascin C is a factor expressed highly in the tumorassociated matrix it would be a desirable first step for targeting the tumorspecific microenvironment In fact a high level of tenascin C expression has been reported in most solid tumors including lung cancer colon cancer and glioblastoma Therefore the targeted binding of tenascin C in tumor stroma would inhibit tumor metastasis by modulating cancer cell growth and migration We isolated a peptide that bound to tenascin C by phage display peptide library selection and the selected peptide specifically recognized tenascin C protein in xenograft mouse tissue We also observed exclusive staining of tenascin C by the selected peptide in tumor patient tissues Moreover the peptide reduced tenascin Cinduced cell rounding and migration We propose that the tenascin C targeting peptide may be useful as a specific anticancer diagnostic and therapeutic tool for most human solid tumors

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