The establishment of mouse embryonic stem cell cul

Authors: Meeyoung Cho TaeJun Cho Jeong Mook Lim Gene Lee Jaejin Cho

Publish Date: 2013/05/16

Volume: 35, Issue: 5, Pages: 456-461

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Abstract

Embryonic stem ES cells can be valuable for monitoring differentiation processes and for improving applications in basic developmental biology The application of ES cells can be a useful tool for drug discovery and toxicology Therefore we suggest the highthroughput screening HTS system based on ES cells in this study Firstly we optimized the feederfree condition and seeding cell number which can maintained for at least 7 days without overconfluency We analyzed the system by cell viability proliferation activity RTPCR and morphologic/immunohistochemical evaluations The optimal cell seeding number was 30/well that was maintained the typical colonial morphology over 9 d with 1000 U/ml LIF in the limited space The cell in optimized condition expressed ALP SSEA1 Oct 4 and Nanog and the genetic expressions showed similar to protein expressions The cell lineage marker expressions showed faint or none The cell viability and proliferation activity were increased in timedependent manner in our optimized HTS system In conclusion the novel HTS system using ES cells can by useful for developing models for drug discovery as well as toxicological screening in the near future

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