Curcumin induces apoptosis in human colorectal car

Authors: Adeeb Shehzad Jaetae Lee TaeLin Huh Young Sup Lee

Publish Date: 2013/05/16

Volume: 35, Issue: 6, Pages: 526-532

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Abstract

Curcumin diferuloylmethane the yellow pigment of turmeric is one of the most commonly used and extensively studied phytochemicals due to its pleiotropic effects in several human cancers In the current study the therapeutic efficacy of curcumin was investigated in human colorectal carcinoma HCT15 cells Curcumin inhibited HCT15 cells proliferation and induced apoptosis in a dose and timedependent manner Hoechst 33342 and DCFHDA staining revealed morphological and biochemical features of apoptosis as well as ROS generation in HCT15 cells treated with 30 and 50 μM curcumin Overexpression of premRNA processing factor 4B Prp4B and p53 mutations have been reported as hallmarks of cancer cells Western blot analysis revealed that curcumin treatment activated caspase3 and decreased expression of p53 and Prp4B in a timedependent manner Transfection of HCT15 cells with Prp4B clone perturbed the growth inhibition induced by 30 μM curcumin Fractionation of cells revealed increased accumulation of Prp4B in the nucleus following its translocation from the cytoplasm To further evaluate the underlying mechanism and survival effect of Prp4B we generated siRNAPrp4B HCT15 clones Knockdown of Prp4B with siRNA diminished the protective effects of Prp4B against curcumininduced apoptosis These results suggest a possible underlying molecular mechanism in which Prp4B overexpression and activity are closely associated with the survival and regulation of apoptotic events in human colon cancer HCT15 cells

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