Authors: Yu Yu Liu Wei Min Yao Tie Wu Bi Lian Xu Fang Chen Liao Cui
Publish Date: 2010/08/06
Volume: 29, Issue: 2, Pages: 149-158
Abstract
The present study was designed to investigate the effects of captopril an angiotensinconverting enzyme inhibitor ACEI on bone loss in aged ovariectomized OVX rats and its impact on the differentiation of cultured primary osteoblasts Tenmonthold female Sprague–Dawley rats were used for the study After 2 months post ovariectomy OVX the rats were treated with captopril 1 or 5 mg/kg/day respectively for another 2 months At endpoint trabecular bone of the fourth lumbar vertebrae L4 was undecalcified and examined by bone histomorphometry the fifth lumbar vertebrae L5 were examined by compression test Primary osteoblasts were isolated from the calvaria of newborn rats and treated with different concentrations of captopril in a different durations The content of secreted alkaline phosphatase ALP and mRNA expression of collagen I in osteoblasts were determined to demonstrate osteoblast bone formation In aged rats with estrogen deficiencyinduced osteopenia captopril increased the trabecular area BV/TV of L4 up to 33 and improved biomechanical properties by increasing L5 break stress and elastic modulus when compared to those in the OVX group P 001 Captopril showed dosedependent effects on promoting the secretion of ALP and increased mRNA expression of collagen I in the cultured rat osteoblasts In summary captopril one of the most widely used ACEIs has the potential effects of improving lumbar vertebral bone strength in aged OVX rats and promoting osteoblast bone formation in vitro
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