Authors: Daiki Morimoto Tetsuya Tomita Shoko Kuroda Chikahisa Higuchi Shinichi Kato Toshikazu Shiba Hironori Nakagami Ryuichi Morishita Hideki Yoshikawa
Publish Date: 2010/02/10
Volume: 28, Issue: 4, Pages: 418-423
Abstract
The existence of inorganic polyphosphates polyP in human cells has been demonstrated In osteoblasts it is suggested that the concentration of cellular polyP is relatively high In this study we examined whether polyP accelerates the differentiation of human mesenchymal stem cells hMSCs from patients with osteoarthritis OA and rheumatoid arthritis RA into osteoblastic cells Alkaline phosphatase ALP activity was induced by polyP in hMSCs from both OA and RA In Alizarin Red S and osteocalcin EIA there was a significant difference between the control and polyP group In realtime PCR there was a significant difference in ALP collagen type 1A osteocalcin and bone sialoprotein between the control and polyP group Our findings suggest that polyP have the potent role of differentiating hMSCs into osteoblastic cells at the early and later stages of osteoblastic differentiation
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