Authors: Andrea S Doria Adrian Crawley Harpal Gahunia Rahim Moineddin Tammy Rayner Vivian Tassos Anguo Zhong Kenneth Pritzker Maria Mendes Roland Jong Robert B Salter
Publish Date: 2011/08/05
Volume: 42, Issue: 1, Pages: 63-75
Abstract
Rabbit knees were injected with albumin n = 9 or saline n = 6 intraarticularly or were not injected control knees n = 9 Except for two rabbits albumininjected n = 2 knees salineinjected n = 2 knees that unexpectedly died on days 7 and 21 of the experiment respectively all other animals were scanned with BOLD MRI on days 0 1 7 14 21 and 28 after induction of arthritis T2weighted gradientecho MRI was performed during alternate 30 s of normoxia/hyperoxia BOLD MRI measurements were compared with clinical laboratory and histological markersPercentage of activated voxels was significantly greater in albumininjected knees than in contralateral salineinjected knees P = 004 For albumininjected knees P 005 and among different categories of knees P = 0009 the percentage of activated BOLD voxels varied over time A quadratic curve for onandoff BOLD difference was delineated for albumin and salineinjected knees over time albumininjected P = 0047 salineinjected P = 0009 A trend toward a significant difference in synovial histological scores between albumininjected and salineinjected knees was noted only for acute scores P = 007The authors thank Michael Noseworthy for setting up the MR sequences used in this study and for reviewing the first draft of the manuscript Sharon Nancekivell for editorial assistance Dr Brian Feldman for advice about the design of the study Dr Geraldine Kent for help with the preparation of the albumin solution Marianne Rogers for histological services Jeff Alfonsi and Niels Celeghin for analysis of data Man Khun Chan and Dr Adele Khoskow for suggesting and testing laboratory surrogates for inflammation in rabbits and Hillary Bruce and Mary Ancona for collaborating as MR imaging technologists in part of the study
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