Authors: O Miriam RutiagaQuiñones Érica Córdova María A MartellNevárez Jóse M Guillamón Nicolas Rozès Jesús Páez
Publish Date: 2012/04/26
Volume: 235, Issue: 1, Pages: 29-35
Abstract
Nitrogen has a significant effect on the fermentation rate and the chemical composition of alcoholic beverages Nitrogen deficiency during fermentation of Agave juice for mescal production can lead to slow fermentations and endproducts with low aromatic compound variety In this study the effects of NH4Cl supplementation on volatile compound formation in Agave duranguensis juice fermented at 28 °C with the native yeast strains Saccharomyces cerevisiae ITD00185 Hanseniaspora uvarum ITD00108 Torulaspora delbrueckii ITD00110 and Kluyveromyces marxianus ITD00211 were analysed Nitrogen content in the Agave juice unsupplemented with NH4Cl was low In the control treatments the four yeasts consumed nitrogen at approximately the same rate almost completely finishing by 24 h Nitrogen supplementation increased biomass production with S cerevisie H uvarum and T delbrueckii but not with K marxianus K marxianus consumed the total assimilable nitrogen more slowly than the other strains in the supplemented fermentations In addition the volatile compound profile differed between the studied yeasts Volatile compound production by S cerevisiae H uvarum and K marxianus was higher in the supplemented fermentations compared to the unsupplemented ones In T delbrueckii the initial volatile compound concentrations remained unchanged or decreased for some compounds with nitrogen supplementation The initial acetic acid and vanillin concentrations decreased with all strains tested and nitrogen supplementation Furthermore the concentration of higher alcohols increased with S cerevisiae and H uvarum in the NH4Clsupplemented fermentations but they decreased with T delbrueckii and K marxianus In conclusion the addition of an inorganic nitrogen source promotes microorganism metabolism increases biomass formation and benefits the fermentation processThis research was supported by the Consejo Nacional de la Ciencia y Tecnología CONACYT We also want to thank the Instituto de Agroquímica y Tecnología de los Alimentos CSIC Departamento de Biotecnología de los Alimentos and the Facultad de Enologia Universitat Rovira i Virgili Departamento de Bioquimica y Biotecnologia
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