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Title of Journal: Eur Food Res Technol

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Abbravation: European Food Research and Technology

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Springer Berlin Heidelberg

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DOI

10.1016/0301-0104(79)85128-9

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ISSN

1438-2385

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Metabolomic and chemometric study of Emphasis Typ

Authors: Susmita Das Mainak Dutta Koel Chaudhury Bratati De
Publish Date: 2015/11/14
Volume: 242, Issue: 5, Pages: 733-743
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Abstract

Achras sapota L fruit extracts have previously been reported to have activities against the carbohydratedigesting enzymes αamylase and αglucosidase The present work was aimed to identify the inhibitors of αamylase and αglucosidase using metabolomics and chemometric approaches Metabolite profile of Achras sapota fruit extracts at different stages of postharvest ripening was analyzed using GC–MSbased metabolomics approach The extracts were also assayed for their properties to inhibit the carbohydratedigesting enzymes αamylase and αglucosidase in vitro The fruit extracts just after harvesting showed maximum activities against the enzymes αamylase and αglucosidase The activities declined with postharvest ripening It was observed from the chemometric analysis that gallic acid chlorogenic acid benzene124triol and dmalic acid are responsible for high αamylase inhibitory activity Gallic acid and chlorogenic acid are positively correlated with high αglucosidase inhibitory activity Inhibition of the two enzymes by gallic acid benzene124triol chlorogenic acid and αamylase by dmalic acid supported the findings The present experiment reports the importance of both primary and secondary metabolites for the bioactivity of plant extractsThe authors are grateful to the Agricultural Field Station of University of Calcutta Baruipur Kolkata India for supplying the cricket ball variety of fruits SD acknowledges financial assistance from University Grant Commission India Faculty Development Programme—Teacher Fellowship Award Financial support from DST FIST is also acknowledged

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