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Title of Journal: Eur Food Res Technol

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Abbravation: European Food Research and Technology

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Springer-Verlag

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DOI

10.1007/s10856-014-5182-8

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1438-2385

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Highthroughput detection of genetically modified

Authors: Feng Xia Luan Ran Tao Yi Gang Xu Jing Wu Xue Jia Guan
Publish Date: 2012/02/02
Volume: 234, Issue: 4, Pages: 649-654
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Abstract

The aim of this study was to develop a method for simultaneous detection of a variety of genetically modified GM rice ingredients in foods using multiplex polymerase chain reaction PCR coupled with highperformance liquid chromatography HPLC assay The following exogenous genes found in GM rice were selected as targets CaMV35S NOS Cry1Ac Bar and Xa21 The endogenous gene PEPCex of rice was selected as an internal control In brief six pairs of primers for multiplex PCR were designed according to the specific region of CaMV35S NOS Cry1Ac Bar Xa21 and PEPCex and following the optimization a multiplex PCR assay was developed and then the multiplex PCR products were subjected to HPLC analysis The GM rice lines ShanYou 63 KeFeng 6 KangYou 97 and LLrice 62 were used as reference GM rice samples to evaluate the potential diagnostic capability of the method Results demonstrated that the multiplex PCRHPLC developed in this work was an efficient diagnostic method for simultaneous identification of the target genes with 015 ng/mL of high sensitivity suggesting a better alternative for the rapid detection of many genetic modification events

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