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Title of Journal: Eur Food Res Technol

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Abbravation: European Food Research and Technology

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Springer-Verlag

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DOI

10.1016/0006-2952(93)90414-r

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1438-2385

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Detection of histamineproducing bacteria using po

Authors: Rita Alves Ana dos Santos Maria Martins
Publish Date: 2014/02/07
Volume: 214, Issue: 2, Pages: 178-180
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Abstract

The polymerase chain reaction technique was used to enable the detection of DNA specific amplicons corresponding to gene fragments coding for histidine decarboxylase present in the histidine decarboxylating bacteria frequently found in canned fish The level of histamine in several fish product samples was quantified by HPLC and the DNA of the samples containing histamine was isolated and amplified with hdcA histidinedecarboxylase genespecific primers The primer sets used CL1/CL2 JV16HC/JV17HC and CL1/JV17HC amplified 150 bp 370 bp and 500 bp products respectively Nonexpected fragments 100 bp 150 bp and 250 bp were also amplified by JV16HC/JV17HC Some amplified fragments were sequenced automatically presenting high homologies with the Clostridium perfringens hdcA gene A DNA probe from a C perfringens pure strain was hybridized with the DNA fragments amplified from the contaminated samples The hybridization blots were then detected by colorimetry revealing that the samples were contaminated by C perfringens

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