Authors: Tomasz Girek Tomasz Goszczyński Beata Girek Wojciech Ciesielski Janusz Boratyński Piotr Rychter
Publish Date: 2012/03/04
Volume: 75, Issue: 3-4, Pages: 293-296
Abstract
The design of proteins whose structure and function can be manipulated by binding with specific ligands such as cyclodextrins has been of great interest in the field of protein engineering and also could be used as drug delivery systems in targeted cancer therapy Loftsson and Duchêne Int J Pharm 3291–11 1 Loftsson et al Expert Opin Drug Deliv 2335–351 2 CD/proteins conjugates are synthesized using original high temperature method in which mono6OformylβCD reacts with two proteins basic pancreatic trypsin inhibitor and lysozyme The proposed synthesis method has a high reproducibility which makes it useful for pharmaceutical purposes That method allows to obtain the conjugate without losing protein’s biological and enzymatic activity which will used in the reaction and without violating the chemical structure of cyclodextrin molecules
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